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anti myhc antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti myhc antibody
    Anti Myhc Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myhc+antibody/Myosin+Heavy+Chain+Antibody/pmc13049293-210-9-12
    Average 96 stars, based on 486 article reviews
    anti myhc antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Morroniside ameliorates inflammatory skeletal muscle atrophy via inhibiting canonical and non-canonical NF-κB and regulating protein synthesis/degradation.
    Article Snippet: Immunoblotting was carried out using antibodies to TNFα (1:1000, Cell Signaling, catalog 11948T), RelA (1:1000, Cell Signaling, catalog 8242), p50 (1:1000, Cell Signaling, catalog 13586S), RelB (1:1000, Cell Signaling, catalog 10544S), p52 (1:500, Santa Crus, catalog sc-7386), MyHC (1:500, R&D, catalog MAB4470) and GAPDH (1:3000, Goodhere, catalog AB-P-R 001). .. For ubiquitination assay, 500 μg of protein lysates with 103 nM Ubiquitin-Aldehyde (South Bay Bio, CA, United States, catalog SBB-PS0031) were incubated with MyHC antibody (1:500, R&D, catalog MAB4470), and precipitated proteins were Western-blotted using an Ub antibody (santa cruz, catalog sc-8017). .. Bands were visualized using ECL chemiluminescence (Thermo Fisher, catalog 34577).

    Incubation:

    Article Title: Morroniside ameliorates inflammatory skeletal muscle atrophy via inhibiting canonical and non-canonical NF-κB and regulating protein synthesis/degradation.
    Article Snippet: Immunoblotting was carried out using antibodies to TNFα (1:1000, Cell Signaling, catalog 11948T), RelA (1:1000, Cell Signaling, catalog 8242), p50 (1:1000, Cell Signaling, catalog 13586S), RelB (1:1000, Cell Signaling, catalog 10544S), p52 (1:500, Santa Crus, catalog sc-7386), MyHC (1:500, R&D, catalog MAB4470) and GAPDH (1:3000, Goodhere, catalog AB-P-R 001). .. For ubiquitination assay, 500 μg of protein lysates with 103 nM Ubiquitin-Aldehyde (South Bay Bio, CA, United States, catalog SBB-PS0031) were incubated with MyHC antibody (1:500, R&D, catalog MAB4470), and precipitated proteins were Western-blotted using an Ub antibody (santa cruz, catalog sc-8017). .. Bands were visualized using ECL chemiluminescence (Thermo Fisher, catalog 34577).

    Western Blot:

    Article Title: Morroniside ameliorates inflammatory skeletal muscle atrophy via inhibiting canonical and non-canonical NF-κB and regulating protein synthesis/degradation.
    Article Snippet: Immunoblotting was carried out using antibodies to TNFα (1:1000, Cell Signaling, catalog 11948T), RelA (1:1000, Cell Signaling, catalog 8242), p50 (1:1000, Cell Signaling, catalog 13586S), RelB (1:1000, Cell Signaling, catalog 10544S), p52 (1:500, Santa Crus, catalog sc-7386), MyHC (1:500, R&D, catalog MAB4470) and GAPDH (1:3000, Goodhere, catalog AB-P-R 001). .. For ubiquitination assay, 500 μg of protein lysates with 103 nM Ubiquitin-Aldehyde (South Bay Bio, CA, United States, catalog SBB-PS0031) were incubated with MyHC antibody (1:500, R&D, catalog MAB4470), and precipitated proteins were Western-blotted using an Ub antibody (santa cruz, catalog sc-8017). .. Bands were visualized using ECL chemiluminescence (Thermo Fisher, catalog 34577).

    In Vitro:

    Article Title: Atrazine exposure induces skeletal muscle atrophy: Multi-omics insights into mechanisms and therapeutic targets.
    Article Snippet: These were incubated overnight at 4 ◦C, followed by the appropriate Alexa Fluor 568‐ or 488‐conjugated goat anti‐mouse IgG1/ IgM (1:400, Invitrogen, A21124/A21042) for 1 h in the dark at RT. .. For in vitro C2C12 myotube detection, MyHC antibody (1:100, R&D Systems, MAB4470) was similarly applied overnight at 4 ◦C, followed by secondary antibody (Alexa Fluor 488‐conjugated goat anti-mouse IgG, 1:200, Invitrogen, A11001) for 1 h at RT in the dark. .. Slides were mounted in VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200–10) and imaged using an Olympus FV3000 inverted confocal microscope.



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    Fibre type-specific myosin heavy chain expression and intramyocellular lipid content in four human skeletal muscles from control and T2DM subjects. Expression of MyHC isoforms type 1, type 2a, type 2x, and intramyocellular lipid visualised by Sudan Black in successive transverse sections of the splenius capitis (SC), diaphragm (DIA), external intercostal (EXT) and vastus lateralis (VL) muscles. Within each group, panels are arranged from left to right as follows: BA-D5 (MyHC-1-positive fibres, including type 1 and 1/2a hybrids), SC-71 (MyHC-2a-positive fibres, including type 2a and hybrid fibres), <t>6H1</t> (MyHC-2x-positive fibres, including type 2x and 2a/2x hybrids), and Sudan Black B (IMCL, intramyocellular lipid). Fibre types and hybrids were assigned by matching fibres across serial BA-D5, SC-71 and 6H1 sections and interpreting staining intensity patterns, with hybrids defined by concomitant positivity on two antibodies, specifically BA-D5 plus SC-71 for type 1/2a and SC-71 plus 6H1 for type 2a/2x, according to established MyHC-based fibre typing conventions. Scale bar = 100 μm. DIA, diaphragm; SC, splenius capitis; EXT, external intercostal; VL, vastus lateralis; CTRL, control; DM, diabetes mellitus (type 2)
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    Image Search Results


    Fibre type-specific myosin heavy chain expression and intramyocellular lipid content in four human skeletal muscles from control and T2DM subjects. Expression of MyHC isoforms type 1, type 2a, type 2x, and intramyocellular lipid visualised by Sudan Black in successive transverse sections of the splenius capitis (SC), diaphragm (DIA), external intercostal (EXT) and vastus lateralis (VL) muscles. Within each group, panels are arranged from left to right as follows: BA-D5 (MyHC-1-positive fibres, including type 1 and 1/2a hybrids), SC-71 (MyHC-2a-positive fibres, including type 2a and hybrid fibres), 6H1 (MyHC-2x-positive fibres, including type 2x and 2a/2x hybrids), and Sudan Black B (IMCL, intramyocellular lipid). Fibre types and hybrids were assigned by matching fibres across serial BA-D5, SC-71 and 6H1 sections and interpreting staining intensity patterns, with hybrids defined by concomitant positivity on two antibodies, specifically BA-D5 plus SC-71 for type 1/2a and SC-71 plus 6H1 for type 2a/2x, according to established MyHC-based fibre typing conventions. Scale bar = 100 μm. DIA, diaphragm; SC, splenius capitis; EXT, external intercostal; VL, vastus lateralis; CTRL, control; DM, diabetes mellitus (type 2)

    Journal: Histochemistry and Cell Biology

    Article Title: Fibre morphology, intramyocellular lipid content and 3D capillary architecture in human postural, respiratory and locomotor muscles in type 2 diabetes mellitus

    doi: 10.1007/s00418-026-02477-7

    Figure Lengend Snippet: Fibre type-specific myosin heavy chain expression and intramyocellular lipid content in four human skeletal muscles from control and T2DM subjects. Expression of MyHC isoforms type 1, type 2a, type 2x, and intramyocellular lipid visualised by Sudan Black in successive transverse sections of the splenius capitis (SC), diaphragm (DIA), external intercostal (EXT) and vastus lateralis (VL) muscles. Within each group, panels are arranged from left to right as follows: BA-D5 (MyHC-1-positive fibres, including type 1 and 1/2a hybrids), SC-71 (MyHC-2a-positive fibres, including type 2a and hybrid fibres), 6H1 (MyHC-2x-positive fibres, including type 2x and 2a/2x hybrids), and Sudan Black B (IMCL, intramyocellular lipid). Fibre types and hybrids were assigned by matching fibres across serial BA-D5, SC-71 and 6H1 sections and interpreting staining intensity patterns, with hybrids defined by concomitant positivity on two antibodies, specifically BA-D5 plus SC-71 for type 1/2a and SC-71 plus 6H1 for type 2a/2x, according to established MyHC-based fibre typing conventions. Scale bar = 100 μm. DIA, diaphragm; SC, splenius capitis; EXT, external intercostal; VL, vastus lateralis; CTRL, control; DM, diabetes mellitus (type 2)

    Article Snippet: Serial 10-μm transverse sections were incubated with normal rabbit serum (1:40 in phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA]) to block non-specific binding, followed by incubation with monoclonal antibodies against MyHC isoforms: BA-D5 (MyHC-1), SC-71 (MyHC-2a) and 6H1 (MyHC-2x) (Developmental Studies Hybridoma Bank, Iowa City, IA, USA), each diluted 1:100 in PBS (pH 7.4).

    Techniques: Expressing, Muscles, Control, Staining